Journal:

Article Title: Gain-of-function mutation in the KCNMB1 potassium channel subunit is associated with low prevalence of diastolic hypertension

doi: 10.1172/JCI200420347

Figure Lengend Snippet: Plots of conductance versus voltage for BK channel currents at different Ca2+ concentrations. BK channel currents were recorded from patches excised from cells expressing α (A), α+β1WT (B), α+β1E65K (C), and α+β1WT+β1E65K (D) channel subunits. Normalized conductance was obtained for each test potential from the peak tail current at –80 mV measured at 0 (circles), 100 nM (squares), 500 nM (triangles), and 10 μM (inverted triangles) Ca2+. Solid curves represent fits to the Boltzmann equation. The data at 0 and 100 nM Ca2+ were normalized using the value obtained at 500 nM as maximum tail current. To obtain an average G-V curve for each case, individual curves were displaced along the voltage axis by ΔV = ( – V1/2) (where denotes the average V1/2 for each transfection group). The 300–360 resulting points were then reduced to 61 with the smoothing function of SigmaPlot (SPSS Inc., Richmond, California, USA).

Article Snippet: The 300–360 resulting points were then reduced to 61 with the smoothing function of SigmaPlot (SPSS Inc., Richmond, California, USA). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 4 caption a7 Plot of V 1/2 versus Ca 2+ concentration. (A) V 1/2 versus Ca 2+ concentration obtained for α+β 1WT ( n = 13) and α+β 1E65K ( n = 15). (B) V 1/2 versus Ca 2+ concentration for α+β 1WT and α+β 1WT +β 1E65K ( n = 6). (C) V 1/2 versus Ca 2+ concentration for α ( n = 3).

Techniques: Expressing, Transfection